THE ACTIVITY OF ENZYMES OF THE GLUTATHIONE SYSTEM IN ERYTHROCYTES OF SCALY CARP ON THE EFFECT OF PLUMBUM

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Visnyk LNAU: Agronomy 2018 №22(2): 145-147

THE ACTIVITY OF ENZYMES OF THE GLUTATHIONE SYSTEM IN ERYTHROCYTES OF SCALY CARP ON THE EFFECT OF PLUMBUM

Onyskovets М., Candidate of Biological Sciences
Lopotich N., Candidate of Agricultural Sciences
Skab O., Candidate of Agricultural Sciences
Lviv National Agrarian University

https://doi.org/10.31734/agronomy2018.02.145

Annotation

It is known that the main mechanism of Plumbum toxicity is the development of oxidative stress as evidenced by disturbances in the pro-and antioxidant blood system as an integral indicator of the body status. In this regard, important is to ascertain the metabolic effects of Plumbum in red blood cells that are among the first to fall under the influence of toxins altered internal environment and possess strong antioxidant defense system. The purpose of our study was to investigate the effect of Plumbum on the activity of enzymes of the glutathione systemin erythrocytes of scaly carp.

Experiments were performed on 2-year old fishes, which after the catching from natural pond were kept in aquaria under standard laboratory conditions. In our studies we used a two-year carp flake (Cyprinus carpio L.) with average weight 300–350 g. Each experimental group included in 7 animals. We investigated the influence of Plumbum ions (Pb2+) at a concentration of 0,2; 0,5 and 5 mg/l, corresponding to 2; 5 and 50 maximum permissible concentration (MPC) on the fish organism. After a period of acclimization the fishes of studied groups were kept in the presence of Pb(CH3COO)2´3Н2О. As a result of the studies we found a significant increase in the activity of antioxidant enzymes during exposure of 2 and 5 MPC Plumbum ions, and inhibition of enzyme activity at 50 MPC, indicating significant stress protective systems against the background of activation of oxidative stress. As a result of the studies we found a significant increase in the activity of antioxidant enzymes during exposure of 2 and 5 MPC Plumbum, and inhibition of enzyme activity at 50 MPC, indicating significant stress protective systems against the background of activation of oxidative stress.

Key words

Plumbum, erythrocytes, blood, antioxidant system, glutathione peroxidase, glutathione reductase, scaly carp

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