Annotation

Нeat Shock Proteins belong to the natural biomarkers, which are important indicators for animal diseases diagnostics and /or instrument of analyzing the effects on organism of the factors deteriorating the habitat. The contamination of water by heavy metals, especially by lead (even in small quantities), is very dangerous and has adverse effect on fish organism. The analysis of toxic effects of the lead ions on the level of expression of such heat shock proteins as HSP70 and HSC70 family in liver and gills of the scaly carp was the main goal of our investigation. Such investigations are really actual and important for estimation of the fishes health during water biocenosis monitoring.

Necessary levels of heavy metals concentrations in all series of experiment were simulated by adding in the water environment of the pool with fish 0,2; 0,5 and 5 mg/dm³ of lead (in the form of Pb(CH₃COO)₂´3Н₂О). Those concentrations correspond to the 2nd, 5th and 50th-fold indices of the threshold limit value of lead in water environment. Concentration of HSP70 and HSC70 was determinated by the dot-blot-analysis due to application of monoclonal antibodies against heat shock proteins [5A5] (ab2787) (Abcam, USA) and [1B5] (ab19136) (Abcam, USA) and polyclonal goat antimice antibodies, conjugated with alkaline phosphatase («Tropix», USA).

The significant dose-depended increase (p<0,001) in all experimental groups of HSP70 concentrations in liver and gills has been detected, applying dot-blot analysis. At the same time, significant changes in expression of HSC70 protein have not been established. It is the evidence that stress-proteins are the sensitive markers of toxic effects of excessive concentration of lead.