Identification and studyng of antagonistic peculiarities of cellulose fungi-destructantas to the sugar-beet tubers phusariosis root agents

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Visnyk LNAU: Agronomy 2019 №23: 164-169

Identification and studyng of antagonistic peculiarities of cellulose fungi-destructantas to the sugar-beet tubers phusariosis root agents

Karas I., Candidate of Agricultural Scien
ORCID ID: 0000-0001-6958-3636
Nevmerzhytska O., Candidate of Agricultural Scien
ORCID ID: 0000-0003-2024-9316
Plotnytska N., Candidate of Agricultural Scien
ORCID ID: 0000-0001-7758-1307
Pavliuk I., Postgraduate
ORCID ID: 0000-001-9603-4608
Zhutomur National Agroecological University

https://doi.org/10.31734/agronomy2019.01.164

Annotation

The purpose of the research was to find the biological agents for sugar-beets protection against the tubers phusariosis root.

The research was conducted in the period of 2015–2017 at the laboratory of plant protection department ZNAEU. Free fungi cultures such as Fusarium oxysporum, Fusarium graminearum, Fusarium culmorum, Fusarium avenaceum, Fusarium solani, have been used, that were extracted from infected sugar-beets tubers.

The search for microorganismus was conducted by means of leaves testing selection from mixed forest debris layer that further can be used in the biological protection. The extraction of primary fungi cultures was conducted within the medium of Van-Iterson and Chapek.

Approximately 231 isolates belonging to 7 genus such as Penicillium, Fusarium, Aureobasidium, Aspergillus, Verticillium, Trichoderma, and Cladosporium, were extracted from the mixed forest debris of Zhitomir and boarding regions.

Fungi correlation of extracted genus was the following: Penicillium genus amounts to 25, 25 %, Fusarium amounts to 22,3 %, Aspergillus 19,3 %, and Trichoderma14,4 %, that were numerous.

Among the isolates examined the most antagonistic activities as to Fusariumoxysporum species the fungi Trichoderma hamatum has been manifested. Under common cultivation Trichoderma hamatum out of Fusarium oxysporum fungi stasis zone 17,3 mm, the former one was observed.

The research has determined, that ZH-6 strain has an antagonistic action as to the (F. oxysporum, F. gibbosum, F. gavanicum, F.solani) sugar-beets tubers phusariosis root agents.

That is why, ZH-6 strain is expedient to use while developing the biological preparations for sugar-beet tubers protection against phusariosis root, as it has revealed the most antagonistic activity.

Key words

Trichoderma hamatum, sugar beet, the agent, fusarium rot, rot crop, strain

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